by P Koloušková · 2017 · Cited by 5 — An equal amount of total RNA (500 ng) from each tissue sample was reverse transcribed with Transcriptor High Fidelity cDNA synthesis kit ...
transcriptor high fidelity cdna synthesis kit pdf download
were used for reverse transcription with the Transcriptor High Fidelity cDNA. Synthesis Kit (Roche) following the manufacturer's instructions. SERAC1 cDNA .... The Transcriptor First Strand cDNA Synthesis Kit is designed for first-strand cDNA ... which produce high quality intact RNA templates suitable for RT-PCR, please refer to our Special Interest Site on Manual Nucleic Acid Isolation and Purification at ... Visit lifescience.roche.com, to download or request copies of the following .... PDFDownload PDFCopy Link CiteAll Options ... The 16S rRNA transcript:gene copy ratio confirmed this assumption and pointed toward a ... following the manufacturer's instructions for the MoBio UltraClean Soil DNA Isolation kit ... µg of total RNA‐DNase using Transcriptor High Fidelity cDNA Synthesis Kit according to the .... by V Geißler · 2013 · Cited by 33 — pathway, Upf proteins, a set of conserved factors of which Upf1 is the central ... nanojuice (Merck) according to the user manual and ... Fidelity cDNA Synthesis Kit (Roche). ... primers and the Transcriptor High Fidelity cDNA.. SMARTer™ PCR cDNA Synthesis Kit User Manual ... transcription is complete. ... Barnes, W. M. (1994) PCR amplification of up to 35-kb DNA with high fidelity .... mMESSAGE mMACHINE kit (Ambion). ... were synthesized using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche). ... Click here to Download Table S1.. by Y Wang · 2013 · Cited by 20 — transcription of FD-dependent floral meristem identity genes such as APETALA1 ... with AccuScript High-Fidelity cDNA Synthesis Kit (Agilent.. by BM Obeng · 2020 — ... generate complementary DNA (cDNA) of HIV-1 from extracted RNA using Transcriptor High Fidelity cDNA synthesis kit (Roche Diagnostics, .... Transcriptor Reverse Transcriptase is recommended for RT-PCR because of its high sensitivity in combination with high thermostability: the enzyme synthesizes .... Transcriptor High Fidelity cDNA Synthesis Kit –. Accuracy Meets RT-PCR. Renate Kolb, Waltraud Ankenbauer, and Brigitte Hloch*. Roche Applied Science .... by M Cartolano · 2016 · Cited by 38 — The first strand cDNA:RNA hybrid molecules were 5-fold diluted and PCR amplification with the KAPA HiFi polymerase (KAPA HiFi PCR kit, .... by J Pramanik · 2018 · Cited by 22 — cDNA was prepared by annealing 500 ng RNA with oligo dT as per the man- ufacturer's instructions (Transcriptor High Fidelity. cDNA Synthesis kit, Roche).. by T Müller · 2021 — tor receptor (EGFR) using a set of TPC knockout cells. ... into cDNA using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche). EGFR and .... by M Chiba · 2013 — Metrics: Total PDF Downloads: 967 (Spandidos Publications: 824 | PMC Statistics: 143 ) ... Reverse-transcription polymerase chain reaction (RT-PCR) is a method ... Gene, Tokyo, Japan), according to the manufacturer's instructions. ... the High Capacity cDNA Reverse Transcriptase kit (Life Technologies), .... PCR-511 / Reverse Transcription / RT-PCR cDNA Synthesis. ... The kit is optimized for high efficiency in a broad range of primer-template combinations. SCRIPT .... SensiFAST cDNA Synthesis Kit. 22 ... High Fidelity DNA Polymerases. VELOCITY ... Product sheets and guides are available online either to download or to order at info@meridianlifescience.com ... templates following reverse transcription with the SensiFAST cDNA ... manual processing and straightforward integration with.. by M Poruba · 2019 · Cited by 4 — Liver was used for the subsequent mRNA and protein content analysis. ... of cDNA using a Transcriptor High Fidelity cDNA synthesis kit (Roche .... by KS Reid-Bayliss · 2017 · Cited by 20 — Article · Figures & SI · Info & Metrics · PDF ... Each indexed cDNA is then amplified by high-fidelity PCR and sequenced on an ... By increasing the length of the rolling-circle reverse transcription reaction, we ... RNA libraries were prepared via the ARC-seq protocol, as detailed in SI Materials and Methods.. by R Hayashi · 2017 · Cited by 2 — Download Fulltext PDF ... Using a Transcriptor High Fidelity cDNA Synthesis Kit (Roche), cDNA was reverse transcribed by the Gene Amp PCR .... The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 g of total RNA to single-stranded .... by F Di Giallonardo · 2013 · Cited by 63 — fold higher in vitro recombination rate can be observed in RT-PCR compared to DNA PCR ... Analysis by ShoRAH and manual inspection revealed an in vitro ... (Roche), Transcriptor High Fidelity cDNA Synthesis Kit (Roche),.. High-Capacity cDNA Reverse Transcription Kits Protocol. 3. To view, download, or print the document of interest: a. Right-click the document title. b. Select:.. by A Balato · 2014 · Cited by 19 — cDNA was prepared (Transcriptor High Fidelity cDNA. Synthesis; Roche ... the manufacturer's instructions. ... RNA extraction, cDNA synthesis and quantitative reverse transcription ... The Vectastain Elite ABC Kit (Vector Laboratories,.. Read the Safety Data Sheets (SDSs) and follow the handling instructions. ... The Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit uses the .... The iScript Select cDNA Synthesis Kit is a sensitive reverse transcription kit optimized for RT-PCR ... High-Fidelity Reverse Transcriptase Kit with Flexible Priming Options ... reverse transcriptases according to manufacturer's instructions.. by M Kristen · 2020 · Cited by 3 — Abstract: Reverse transcription of RNA templates containing modified ribonucleosides ... modification-specific treatment is combined with high-performance ... to the reaction buffer, thus lowering sequence-fidelity of the polymerase [26]. ... conditions, multiple polymerases and an extended RT feature set.. 2.0 Reverse Transcription . ... 5.11 qScript microRNA cDNA Synthesis Kit .... 56 ... standard instructions, ... 3'-exonuclease proof-reading polymerase supports high-fidelity downstream applications ... A list of primers for more than 1500 miRNAs have already been designed and are available for download at www.quantabio.. Jan 24, 2016 — 2 Citations; 3k Downloads ... Download protocol PDF ... Transcriptor High Fidelity cDNA Synthesis kit (includes PCR grade water, Random .... by T Conrad · 2020 · Cited by 4 — Second-strand cDNA synthesis was performed in 50 µl with 0.5 µM Oligo i5_5N_dT20VN, 2000 U Q5® High-Fidelity DNA Polymerase, and 0.2 .... by MA Aguilar-Luis · 2020 · Cited by 6 — First, the Transcriptor High Fidelity cDNA Synthesis Kit (Roche. Applied Science, Mannheim, Germany) was used according to the manufacturer's instructions: .... COMBAT NET RADIO TM 11-5820-890-10-6 TECHNICAL MANUAL SINCGARS GROUND ICOM RADIOS Used with ... Transcriptor High Fidelity cDNA Synthesis Kit – Accuracy . ... To view, download, or print the document of interest: a.. Jan 2, 2020 — Treatment protocol, HeLa cells were transfected with 10nM control or USP36 ... mini kit (Qiagen, Germany) following the manufacturer's instructions and 600 ng of RNA were reverse transcribed using the Transcriptor High Fidelity cDNA Synthesis Kit ... Supplementary file, Size, Download, File type/resource.. by Á Baross · 2004 · Cited by 39 — reverse transcription-polymerase chain reaction (RT-PCR)-based approach for ... high-fidelity DNA polymerase (Methods) and gene-specific prim- ers (Fig. 1).. Available as a standalone enzyme, a complete reverse transcription kit or as a master mix with Oligo(dT) or Random Primers; Sensitive transcription of both high-copy and low-copy ... transcribed using oligo(dT) primer according to the manufacturer's instructions. ... Download SDSPDF (234 KB) – English (United States) .... a CREB1 (phospho-S133) Transcription Factor Assay Kit. (Abnova Corp, Taipei City, ... sized with the Transcriptor High Fidelity cDNA Synthesis. Kit (Roche .... May 15, 2007 — 6. Figure 3. Protocol guide for SMART cDNA synthesis for ... for producing high-quality cDNA from nanograms of total or poly A+ RNA. ... who wish to analyze transcript size and expression patterns by hybridization ... Barnes, W. M. (1994) PCR amplification of up to 35-kb DNA with high fidelity and high yield. 4f4a45da30 54
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